Accelerate Protein Expression Feasibility 

What is Pfast?

Pfast™ - Fast Protein Expression Feasibility Analysis
Starting at $25,000, our Pfast™ Feasibility program delivers rapid insights into your protein's potential with the Pfenex platform. De-risk your development pipeline quickly and cost-effectively with ought a full-scale commitment.

How is Pfast different from a full Pfenex program?

Our advanced Pfenex strain engineering program is historically used for extremely challenging proteins or for identified lead candidates where expression optimization is needed. A full platform screen and scale-up is typically 10 to 12 weeks. Pfast accelerates this process using known high-potential toolbox subsets to quickly drive to the optimal expression strategy, or the final strain itself.

The Pfast Workflow

Experience the simplicity and efficiency of Pfast:

Days 1–2

Pooled Cloning of single gene

• 25–200 plasmid pool ligated with gene of interest
• Transform and generate pooled seed culture

Days 3-6

Plate Pooled Culture

• Automated 384 place selective medium plate
• Cherry-pick to 96w growth plate

Days 7-8

HTP Expression

• Grow and induce
• Lyse, rank titer by CGE or ELISA
• ID wells for affinity purification

Days 9-10

Hit Enrichment

• Bead-based HTP IMAC
• CGE: concentration and purity
• Sequence (as needed)

Program Deliverables:

• Detailed protein titer and analysis of soluble and insoluble fractions via SDS-CGE or ELISA.
• Analytical assessments via Western blot and/or BLI, a multitude chromatographic method, and peptide mapping, provided at additional costs.
• Sample material for further testing provided if suitable titers achieved.
• Strategic recommendations for additional strain improvements, or advancement to 2L scale fermentation.

After your Pfast evaluation has completed, further leveraging the Pfenex platform and our know-how is available.