Pfenex Expression Technology Prima RNApols PeliCRM197

Accelerate Protein Expression Feasibility in Just 10 Days

Test the power of the Pfenex Expression Technology® platform with Pfast™ for actionable insights to advance your production requirements.

What is Pfast?

How is Pfast different?

The Pfast Workflow

Pfast Program Deliverables

What is Pfast?

How is Pfast different?

The Pfast Workflow

Pfast Program Deliverables

What is Pfast?

Pfast™ - Rapid Protein Expression Feasibility Analysis
Starting at $25,000, our Pfast™ Feasibility program provides new insights into your proteins potential with the Pfenex platform. Pfast provides a fast, low-cost opportunity to de-risk your development pipeline without committing to a full-scale development program. Empower your progress with confidence.

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How is Pfast different from a full Pfenex program?

Like the name, Pfast is fast. Our advanced Pfenex strain engineering program is historically used when a protein expressed in other platforms has been challenging for a multitude of reasons and developability of the protein is at risk. A full Pfenex platform screen takes 10 - 15 weeks, but Pfast accelerates this process by focusing on high-potential toolbox subsets, delivering faster results more efficiently.

Full Pfenex Evaluation
Pfast™ Feasibility
Combinations screened
Up to 1,000 unique expression strains generated by combining an array of hosts with a set of expression vectors, exploring a number of genetic elements with your gene of interest.
A pooled ligation strategy where your gene of interest is combined with up to 200 different vector combinations, and transformed into one host.
Screening
Duplicate samples of each clone in an automated 96 well format.
Serially diluted cell pool grown in an automated 96 well format.
Purification tags necessary?
No, custom enrichment strategies can be developed.
IMAC compatible or other affinity tag; untagged proteins may incur additional cost.
Analysis
Multitude of parameters measured, including titer, activity (ELISA, BLI enzymatic activity), HPLC, LC-MS,
Titer by SDS-CGE; activity if ELISA or BLI based assay available.
Scale-up
Five parameter, DOE based 2L fermentation assessment of successful clones to select final production strain.
None.
Material provided to client for analysis
Yes, at all stages if applicable.
Yes, if 96 well titer is sufficient.
Costs
Inquire as full evaluation is customized for each target protein based on past experience with similar molecules.
Starting at $25,000.
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The Pfast Workflow

Experience the simplicity and efficiency of Pfast:

Contact Us:

Speak with a Primrose scientist about how Pfast can empower you (CDA not required for initial discussion).

Easy Master Services Agreement:

Contracting for Pfast programs are fast and low-risk, and intended to be.

High-Throughput Screening:

Evaluate 200 vector combinations in one high-performing strain from our vast Pfenex toolbox for titer, and if possible, activity.

Actionable Path Forward:

Receive a comprehensive report detailing results with recommendations for further strain and/or process development.

PFAST FEASIBILITY WORKFLOW
Days 1–2
Pooled Cloning of single gene
  • 25–200 plasmid pool ligated with gene of interest
  • Transform and generate pooled seed culture
Pfast Workflow Image
Days 3-6
Plate Pooled Culture
  • Automated 384 place selective medium plate
  • Cherry-pick to 96w growth plate
Pfast Workflow Image
Days 7-8
HTP Expression
  • Grow and induce
  • Lyse, rank titer by CGE or ELISA
  • ID wells for affinity purification
Pfast Workflow Image
Days 9-10
Hit Enrichment
  • Bead-based HTP IMAC
  • CGE: concentration and purity
  • Sequence (as needed)
Pfast Workflow Image

Program Deliverables:

  • Detailed protein titer and analysis of soluble and insoluble fractions via SDS-CGE or ELISA provided in PowerPoint format.
  • Analytical assessments via Western blot and/or BLI for additional costs. Optional advanced analytics, including HPLC, LC-MS, LC-MS/MS, MALDI-TOF (peptide mapping) and others are performed in-house.
  • Sample material for further testing provided if suitable titers are achieved.
  • Strategic optimization recommendations of identified clone(s) for additional strain improvements, or advancement to 2L scale fermentation.

After your Pfast evaluation has completed, multiple options are available to continue your work leveraging Pfenex platform:

Further Strain Optimization
Results guide how to refine improved expression across multiple facets of additional strain engineering or advancement of the identified clone.
Scale-Up
Transition to 2L and then 30L, or 100L processes development once a stable clone is chosen.
Flexible Licensing
Secure access to your new PET strain via our licensing model, which is industry-friendly with royalty and royalty-free options.

Take the first step toward optimized protein expression. Start Your Pfast Feasibility Study Today.